For Lab 18, we detected the presence of a genetic mutation using gel electrophoresis (from back in Lab 13!). Below is a picture of the gel from Lab 18. Use it to complete your analysis and determine the presence of a mutated gene. For this gel: Lane 1 control - homozygous for mutated allele, has sickle cell anemia; Lane 2 control - heterozygous for mutated allele and wild-type allele, carrier for sickle cell anemia; Lane 3 - homozygous dominant for wild-type alleles, does not have sickle cell anemia; Lane 4 - Mother of Child; Lane 5 - Child; Lane 6 - Father of Child
Use these pictures & data to complete your lab packet observing blood types and applying patterns of inheritances to blood types. If the sample is "positive" for agglutination, then there is a "+" next to the well. If the sample is "negative" for agglutination, then there is a "-" next to the well. For example, sample 1 is negative (-) for agglutination with Anti-A serum and is negative (-) for agglutination with Anti-Rh serum, but it is positive (+) for agglutination with Anti-B serum. Therefore, Sample 1 is blood type B-. Complete your data table in your lab packet and answer all questions.
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